GSM3721570: CB CD34+ cells with FLT3-ITD-Replicate2; Homo sapiens; RN... - SRA

SRX5676602: GSM3721570: CB CD34+ cells with FLT3-ITD-Replicate2; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 40.2M spots, 2.1G bases, 1.1Gb downloads

Submitted by: NCBI (GEO)

Study: PRMT1-mediated FLT3 arginine methylation promotes FLT3-ITD+ Acute Myeloid Leukemia maintenance

PRJNA532633 • SRP192427 • All experiments • All runs

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We want to obtain FLT3-ITD gene signature. To do so, we transduced CB CD34+ cells with mock or FLT3-ITD vectors and performed RNA sequencing (RNA-Seq). Two Groups: Group1: CB CD34+ cells transduced with mock vector; Group2: CB CD34+ cells transduced with FLT3-ITD vector; Overall design: Expression profiling data in FLT3-ITD+ Cord blood cells were compared to corresponding control mock transduced cells by RNA-seq technology

Sample: CB CD34+ cells with FLT3-ITD-Replicate2

SAMN11407998 • SRS4618221 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was extracted using Trizol Reagent (Thermo Fisher Scientific) following the manufacture`s instructions. RNA quality was assessed by spectrophotometry(NanoDrop, ND1000) and gel electrophoresis. To be considered as high-quality, the RNA had to have the result of A260/A280≧1.8, A260/A230≧1.5, no gDNA contamination. RNA integrity was measured by Agilent RNA 6000 Nano Assay (RIN≧6). RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM3721570

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 40.2M spots, 2.1G bases, 1.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR8890939	40,245,101	2.1G	1.1Gb	2020-04-01

ID:: 7650619

SRA

Sequence Read Archive

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